Systems Neuroscience & Neuroengineering
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Research |
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The RoLi lab is the joint lab of Drew Robson and Jennifer Li. We are interested in understanding state-dependent cognition – how an animal’s cognitive model of the world is shaped by its internal neuromodulatory state.
We work with the larval zebrafish, a unique model organism with a compact and transparent brain in which nearly every neuron can be simultaneously recorded. Our neuroengineering team develops novel imaging systems to record and manipulate neural activity in freely swimming larval zebrafish, and our systems neuroscience team use these tools to gain a deeper understanding of the neural mechanisms that underlie internal brain states and spatial cognition. Our lab is a part of the Max Planck Institute for Biological Cybernetics |
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Neuroengineering 
Kim DH, Kim J, Marques JC, Grama A, Hildebrand DGC, Gu W, Li JM* & Robson DN*. Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish. Nature Methods. 2017 Nov;14(11):1107-1114
Over the last decade, larval zebrafish has emerged as an ideal system for brain-wide cellular resolution calcium imaging due to its small nervous system and high optical transparency. However, most existing imaging methods require the animal to be tethered under a microscope, which significantly restricts the range of behaviors the animal can perform. To overcome this limitation, our lab actively develops new microscopy systems that enable whole-brain imaging and manipulation in freely swimming larval zebrafish.
Using these systems, we are now investigating a wide repertoire of natural behaviors, including spatial navigation, social behavior, feeding, and reward. |
Systems Neuroscience 
Marques JCǂ, Li Mǂ, Schaak D, Robson DN* & Li JM*. Internal state dynamics shape brainwide activity and foraging behaviour. Nature 2019. DOI: 10.1038/s41586-019-1858-z
The mammalian brain derived its basic architecture and organization from aquatic fish over 400 million years ago. However, it is far from clear the extent to which the wide array of mammalian cognitive processes and neuromodulatory states are already present in an ancestral form in non-mammalian vertebrate species, and how these ancestral networks may have constrained the evolutionary trajectory of the vertebrate brain.
In our lab, we use the larval zebrafish, an animal with a small transparent brain in which all neurons can be simultaneously recorded at single-cell resolution, to peek backwards in evolutionary time towards the origins of state-dependent cognition. Our current projects include:
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